abstract：AIMS:The medicinal fungi Inonotus xeranticus and Phellinus linteus in the family Hymenochaetaceae have been used as traditional medicines for the treatment of various diseases. However, the compound responsible for the antioxidant activity is still unknown. Therefore, this study was conducted to characterize the antiox...

journal_title：Journal of applied microbiology

authors： Jung JY,Lee IK,Seok SJ,Lee HJ,Kim YH,Yun BS

更新日期：2008-06-01 00:00:00

abstract：AIMS:To better understand and manage the fate and transport of Salmonella in agricultural watersheds, we developed a culture-based, five tube-four dilution most probable number (MPN) method for enumerating dilute densities of Salmonella in environmental waters. METHODS AND RESULTS:The MPN method was a combination of a...

journal_title：Journal of applied microbiology

authors： Jenkins MB,Endale DM,Fisher DS

更新日期：2008-06-01 00:00:00

abstract：AIM:To detect if substances with mammalian cell toxicity are produced by Streptomyces turgidiscabies and Streptomyces scabiei isolated from potato scab lesions. METHODS AND RESULTS:In vitro cultures of phytopathogenic and nonphytopathogenic strains of S. scabiei and S. turgidiscabies, isolated from scab lesions of pot...

journal_title：Journal of applied microbiology

authors： Kotiaho M,Aittamaa M,Andersson MA,Mikkola R,Valkonen JP,Salkinoja-Salonen M

更新日期：2008-05-01 00:00:00

abstract：AIMS:To determine the effect of solar radiation on Cryptosporidium parvum in tap and environmental waters. METHODS AND RESULTS:Outdoor tank experiments and a cell culture infectivity assay were used to measure solar inactivation of C. parvum oocysts in different waters. Experiments conducted on days with different lev...

journal_title：Journal of applied microbiology

authors： King BJ,Hoefel D,Daminato DP,Fanok S,Monis PT

更新日期：2008-05-01 00:00:00

abstract：AIM:Introduction of a protocol for broad-range diagnosis of bacterial infections, which remain negative in culture. METHODS AND RESULTS:The new TaqMan real-time PCR assay amplifies part of the 16S rRNA gene. Species are identified by subsequent sequencing and phylogenetic blast analysis. The analytical sensitivity sho...

journal_title：Journal of applied microbiology

authors： Schabereiter-Gurtner C,Nehr M,Apfalter P,Makristathis A,Rotter ML,Hirschl AM

更新日期：2008-04-01 00:00:00

abstract：AIM:To investigate the role of biofilm-forming Paenibacillus polymyxa strains in controlling crown root rot disease. METHODS AND RESULTS:Two plant growth-promoting P. polymyxa strains were isolated from the peanut rhizosphere, from Aspergillus niger-suppressive soils. The strains were tested, under greenhouse and fiel...

journal_title：Journal of applied microbiology

authors： Haggag WM,Timmusk S

更新日期：2008-04-01 00:00:00

abstract：AIM:A new medium, EC-Blue-10, containing chromogenic and fluorogenic substrates, KNO(3) and sodium pyruvate has been developed for the rapid simultaneous detection and enumeration of total coliforms and Escherichia coli in water. METHODS AND RESULTS:Two evaluations of EC-Blue-10 were carried out. Firstly, EC-Blue-10 w...

journal_title：Journal of applied microbiology

authors： Kodaka H,Mizuochi S,Saito M,Matsuoka H

更新日期：2008-04-01 00:00:00

abstract：AIMS:To determine the major components of total and metabolically active microbial communities of yellow and grey colonizations threatening the conservation of palaeolithic paintings in Altamira Cave (Spain). METHODS AND RESULTS:Micro-organisms present in yellow and grey colonizations were determined from DNA analysis...

journal_title：Journal of applied microbiology

authors： Portillo MC,Gonzalez JM,Saiz-Jimenez C

更新日期：2008-03-01 00:00:00

abstract：AIMS:To understand factors that impact solar-powered electricity generation by Rhodobacter sphaeroides in a single-chamber microbial fuel cell (MFC). METHODS AND RESULTS:The MFC used submerged platinum-coated carbon paper anodes and cathodes of the same material, in contact with atmospheric oxygen. Power was measured ...

journal_title：Journal of applied microbiology

authors： Cho YK,Donohue TJ,Tejedor I,Anderson MA,McMahon KD,Noguera DR

更新日期：2008-03-01 00:00:00

abstract：AIMS:The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT-33, and to investigate the apoptosis effects of its metabolite on colon cancer cells. METHOD AND RESULTS:We isolated 93 marine bacteria from seawater samples. Of these, strain BIT-33 exhibited the strongest cytotoxic acti...

journal_title：Journal of applied microbiology

authors： Jeong SY,Park SY,Kim YH,Kim M,Lee SJ

更新日期：2008-03-01 00:00:00

abstract：AIMS:To develop a real-time PCR assay targeting the Escherichia coli flagellar antigen H21 for identification and surveillance of clinically important Shiga toxin-producing E. coli (STEC) serotypes classified in seropathotype C. METHODS AND RESULTS:The fliC allele of STEC O91:H21 strain B2F1 was amplified and sequence...

journal_title：Journal of applied microbiology

authors： Auvray F,Lecureuil C,Taché J,Perelle S,Fach P

更新日期：2008-03-01 00:00:00

abstract：AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...

journal_title：Journal of applied microbiology

authors： Guo XL,Leng P,Yang Y,Yu LG,Lou HX

更新日期：2008-03-01 00:00:00

abstract：AIMS:The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. METHODS AND RESULTS:This micro-organism, on t...

journal_title：Journal of applied microbiology

authors： Simon-Colin C,Alain K,Colin S,Cozien J,Costa B,Guezennec JG,Raguénès GH

更新日期：2008-02-01 00:00:00

abstract：AIMS:Adenosine triphosphate (ATP) during the enzymatic production of glutathione is necessary. In this study, our aims were to investigate the reason for low glutathione production in Escherichia coli coupled with an ATP regeneration system and to develop a new strategy to improve the system. METHODS AND RESULTS:Gluta...

journal_title：Journal of applied microbiology

authors： Liao X,Deng T,Zhu Y,Du G,Chen J

更新日期：2008-02-01 00:00:00

abstract：AIMS:The purpose of this study was to purify and characterize a bacteriocin produced by Eikenella corrodens A32E2. METHODS AND RESULTS:Peptostreptococcus anaerobius ATCC27337 was used as indicator strain in antagonistic assays for bacteriocin-producing E. corrodens A32E2. Protein extraction was influenced by pH and bu...

journal_title：Journal of applied microbiology

authors： Apolônio AC,Carvalho MA,Bemquerer MP,Santoro MM,Pinto SQ,Oliveira JS,Santos KV,Farias LM

更新日期：2008-02-01 00:00:00

abstract：AIMS:To investigate the involvement of oxidative stress and thiol redox state (TRS) in sclerotial differentiation of Sclerotium rolfsii and Sclerotinia sclerotiorum. METHODS AND RESULTS:Oxidative stress in these fungi was assessed by lipid peroxidation, which was higher in comparison with their nonsclerotiogenic count...

journal_title：Journal of applied microbiology

authors： Patsoukis N,Georgiou CD

更新日期：2008-01-01 00:00:00

abstract：AIMS:The objective of this study was to deduce and analyse equations that best describe the behaviour of faecal bacterial indicators and two decomposition parameters during broiler litter composting. Mathematical models were fitted and the order of rate equations were identified. METHODS AND RESULTS:The Levenburg-Marq...

journal_title：Journal of applied microbiology

authors： Sobratee N,Mohee R,Driver MF,Mudhoo A

更新日期：2008-01-01 00:00:00

abstract：AIMS:To evaluate the behaviour of Shiga toxin-producing Escherichia coli (STEC) O26 strains inoculated in manure-amended soils under in vitro conditions. METHODS AND RESULTS:Four green fluorescent protein (GFP)-labelled STEC O26 strains were inoculated in duplicate (at 10(6) CFU g(-1)) in three different manure-amende...

journal_title：Journal of applied microbiology

authors： Fremaux B,Prigent-Combaret C,Delignette-Muller ML,Mallen B,Dothal M,Gleizal A,Vernozy-Rozand C

更新日期：2008-01-01 00:00:00

abstract：AIMS:To investigate the factors affecting expression and solubilization of Escherichia coli maltodextrin glucosidase in E. coli. METHODS AND RESULTS:Expression level and solubilization of the recombinant E. coli maltodextrin glucosidase was studied in E. coli at different temperatures, in presence of overexpressed Gro...

journal_title：Journal of applied microbiology

authors： Paul S,Chaudhuri TK

更新日期：2008-01-01 00:00:00

abstract：AIMS:The potential use of bifidobacteria as indicators for faecal contamination was studied along a sheep meat production and processing chain. The levels of bifidobacteria were compared with those of Escherichia coli. Total viable counts were followed along the chain (244 samples). METHODS AND RESULTS:Forty-three per...

journal_title：Journal of applied microbiology

authors： Delcenserie V,Loncaric D,Bonaparte C,Upmann M,China B,Daube G,Gavini F

更新日期：2008-01-01 00:00:00

abstract：AIMS:To develop a PCR-denaturing gradient gel electrophoresis (PCR-DGGE) method for the detection and identification of Campylobacter, Helicobacter and Arcobacter species (Epsilobacteria) in clinical samples and evaluate its efficacy on saliva samples from humans and domestic pets. METHODS AND RESULTS:A semi-nested PC...

journal_title：Journal of applied microbiology

authors： Petersen RF,Harrington CS,Kortegaard HE,On SL

更新日期：2007-12-01 00:00:00

abstract：AIMS:To identify dominant bacteria in grain (barley)-fed cattle for isolation and future use to increase the efficiency of starch utilization in these cattle. METHODS AND RESULTS:Total DNA was extracted from samples of the rumen contents from eight steers fed a barley diet for 9 and 14 days. Bacterial profiles were ob...

journal_title：Journal of applied microbiology

authors： Klieve AV,O'Leary MN,McMillen L,Ouwerkerk D

更新日期：2007-12-01 00:00:00

abstract：AIMS:To examine the involvement of N-acyl homoserine lactone (AHL) quorum sensing in alkaline metalloprotease (aprX) promoter regulation in a Pseudomonas fluorescens milk isolate. METHODS AND RESULTS:N-acyl homoserine lactone signals from P. fluorescens strain 395 were separated and detected by thin layer chromatograp...

journal_title：Journal of applied microbiology

authors： Liu M,Wang H,Griffiths MW

更新日期：2007-12-01 00:00:00

abstract：AIMS:To develop descriptive models for the combined effect of temperature (10-40 degrees C) and water activity (0.850-0.980) on the growth of two ochratoxin A producing strains of Aspergillus carbonarius from Greek wine grapes on a synthetic grape juice medium. METHODS AND RESULTS:Fungal growth was measured as changes...

journal_title：Journal of applied microbiology

authors： Tassou CC,Panagou EZ,Natskoulis P,Magan N

更新日期：2007-12-01 00:00:00

abstract：AIMS:To determine the transcriptional responses of Trichophyton rubrum to the artificial substance, PH11B. METHODS AND RESULTS:The broth microdilution assay for antifungal susceptibility testing of dermatophytes was used to measure the minimum inhibitory concentration (MIC) of PH11B. cDNA microarray technology and rea...

journal_title：Journal of applied microbiology

authors： Yu L,Zhang W,Liu T,Wang X,Peng J,Li S,Jin Q

更新日期：2007-12-01 00:00:00

abstract：AIMS:To determine the presence of Vibrio cholerae in different areas of Argentina in three sample types, to determine the composition of planktonic communities in areas at which this pathogen was detected and to characterize the virulence properties and antimicrobial resistance of the recovered environmental isolates. ...

journal_title：Journal of applied microbiology

authors： Fraga SG,Pichel M,Costagliola M,Cecilia M,Jurquiza V,Peressutti S,Caffer MI,Aulet O,Hozbor C,Tracanna BC,de Gamundi AV,Hernández D,Ramírez FC,Akselman R,Binsztein N

更新日期：2007-12-01 00:00:00

abstract：AIM:Campylobacter species are significantly implicated in human gastrointestinal infections. Of 20 species of Campylobacter, C. jejuni, C. coli and C. lari have been considered as the most important causative agents of human infections. In order to better understand the occurrence and epidemiology of these thermophilic...

journal_title：Journal of applied microbiology

authors： Khan IU,Edge TA

更新日期：2007-12-01 00:00:00

abstract：AIM:To determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. METHODS:Putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm....

journal_title：Journal of applied microbiology

authors： Kagkli DM,Vancanneyt M,Vandamme P,Hill C,Cogan TM

更新日期：2007-11-01 00:00:00

abstract：AIMS:The goal of this research was the development of a PCR-based assay to identify important decay fungi from wood of hardwood tree species in northern temperate regions. METHODS AND RESULTS:Eleven taxon-specific primers were designed for PCR amplification of either nuclear or mitochondrial ribosomal DNA regions of A...

journal_title：Journal of applied microbiology

authors： Guglielmo F,Bergemann SE,Gonthier P,Nicolotti G,Garbelotto M

更新日期：2007-11-01 00:00:00

abstract：AIM:To investigate the immunoprotection of three recombinant proteins derived from the Vibrio harveyi outer membrane proteins (OMPs) OmpK, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and their fusion OmpK-GAPDH as vaccine candidates from vibriosis of large yellow croaker (Pseudosciaena crocea). METHODS:The ompK g...

journal_title：Journal of applied microbiology

authors： Zhang C,Yu L,Qian R

更新日期：2007-11-01 00:00:00

abstract：AIM:To develop a probiotic with effectiveness against Aeromonas sp., which was pathogenic to rainbow trout. METHODS AND RESULTS:When Bacillus subtilis AB1, which was obtained from fish intestine, was administered for 14 days to rainbow trout in feed at a concentration of 10(7) cells per gram either as viable, formaliz...

journal_title：Journal of applied microbiology

authors： Newaj-Fyzul A,Adesiyun AA,Mutani A,Ramsubhag A,Brunt J,Austin B

更新日期：2007-11-01 00:00:00

abstract：AIMS:To study the genetic basis of C(4)-dicarboxylate transport (Dct) in relation to symbiotic nitrogen fixation in Mesorhizobium ciceri. METHODS AND RESULTS:A Tn5-induced mutant strain (TL16) of M. ciceri, unable to grow on C(4)-dicarboxylates, was isolated from the wild-type strain TAL 620. The mutant lacked activit...

journal_title：Journal of applied microbiology

authors： Gautam US,Jajoo A,Singh A,Chakrabartty PK,Das SK

更新日期：2007-11-01 00:00:00

abstract：AIM:To evaluate Thermus scotoductus SA-01's ability to reduce Cr(VI) aerobically. METHODS AND RESULTS:T. scotoductus SA-01 is able to reduce Cr(VI) aerobically when grown in a complex organic medium containing Cr(VI) concentrations up to 0.5 mmol l(-1). Suspension of T. scotoductus SA-01 cells also reduced Cr(VI) aero...

journal_title：Journal of applied microbiology

authors： Opperman DJ,van Heerden E

更新日期：2007-11-01 00:00:00

abstract：AIMS:To develop a suite of group-specific, rRNA-targeted oligonucleotide scissor probes for the quantitative detection of the predominant bacterial groups within the ruminal microbial community with the rRNA cleavage reaction-mediated microbial quantification method. METHODS AND RESULTS:Oligonucleotides that complemen...

journal_title：Journal of applied microbiology

authors： Uyeno Y,Sekiguchi Y,Tajima K,Takenaka A,Kurihara M,Kamagata Y

更新日期：2007-11-01 00:00:00

abstract：AIMS:To study the effect of the type of antibiotic used in medicated diets against pathogens and the feeding level on the microbial biodiversity in the rabbit caecum. METHODS AND RESULTS:Three groups of eight does were given a diet unsupplemented (NAB) or with 100 ppm of bacitracin (BAC) or tiamulin (TIA). Litter size...

journal_title：Journal of applied microbiology

authors： Abecia L,Fondevila M,Balcells J,Lobley GE,McEwan NR

更新日期：2007-10-01 00:00:00

abstract：AIM:This study focuses on investigating the molecular and physiological characteristics of Prevotella intermedia after molecular oxygen exposure (MOE) and the effect on drug susceptibility patterns. METHODS AND RESULTS:Samples of P. intermedia were used as parent strains: ATCC25611 and four clinical isolates. Strains ...

journal_title：Journal of applied microbiology

authors： dos Santos SG,Diniz CG,da Silva VL,Souza NC,de Lima FL,Bomfim MR,de Carvalho MA,Farias LM

更新日期：2007-10-01 00:00:00

abstract：AIMS:To analyse the morphological and ultrastructural effects of lipopeptides of cell-free liquid cultures from the antagonistic Bacillus subtilis strains, UMAF6614 and UMAF6639, on the cucurbit powdery mildew fungus, Podosphaera fusca, conidial germination. METHODS AND RESULTS:Butanolic extracts from cell-free supern...

journal_title：Journal of applied microbiology

authors： Romero D,de Vicente A,Olmos JL,Dávila JC,Pérez-García A

更新日期：2007-10-01 00:00:00

abstract：AIMS:To estimate the effect of pH on the structures of bacterial community during fermentation of vegetable wastes and to investigate the relationship between bacterial community dynamics and product distribution. METHODS AND RESULTS:The bacterial communities in five batch tests controlled at different pH values [unco...

journal_title：Journal of applied microbiology

authors： Ye NF,Lü F,Shao LM,Godon JJ,He PJ

更新日期：2007-10-01 00:00:00

abstract：AIMS:To generate stable acid-resistant Bifidobacterium strains isolated from human subjects and characterize the phenotypic changes of the acid-resistant derivatives. METHODS AND RESULTS:The ability of 20 Bifidobacterium strains isolated from human faeces to survive to simulated gastrointestinal transit was evaluated,...

journal_title：Journal of applied microbiology

authors： Collado MC,Sanz Y

更新日期：2007-10-01 00:00:00

abstract：AIMS:To develop and evaluate a novel genotypic test for rapid detection of rifampicin and isoniazid resistance of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates by a multiplex probe array. METHODS AND RESULTS:A multiplex probe array was designed for genotypic test to simultaneously screen the mutations ...

journal_title：Journal of applied microbiology

authors： Zhang SL,Shen JG,Xu PH,Li DX,Sun ZQ,Li L,Yang ZR,Sun Q

更新日期：2007-10-01 00:00:00